Circular 13/2013/TT-BTNMT: Regulations on DNA extraction in Vietnam

15:38, 10/07/2024

This is a notable content in Circular 13/2013/TT-BTNMT of the Ministry of Natural Resources and Environment, stipulating technical procedures and economic-technical norms in the detection of genetically modified organisms by qualitative and quantitative deoxyribonucleic acid analysis methods, issued on March 31, 2014.

Regulations on DNA extraction in Vietnam

To be specific, in the Technical Process and Economic - Technical Norms in detecting genetically modified organisms by qualitative and quantitative Deoxyribonucleic Acid analysis methods issued with Circular 13/2013/TT-BTNMT prescribed according to TCVN 7606:2007 (ISO 21571), the basic principle of DNA extraction includes releasing DNA into a mixed solution of substances and then purifying the DNA from that mixture. The main steps to extract DNA include:

- Preparing tools and chemicals;

- Grinding the sample;

- Extracting DNA;

- Purifying DNA.

There are many methods to extract DNA, and depending on the origin of the samples (animal, plant, or microorganism), there are suitable extraction methods. In this set of economic-technical norms, three DNA extraction methods are applied for animal, plant, and microorganism subjects. To be specific:

- Extracting DNA from biological samples of plant origin based on CTAB (Cetyl trimethylammonium bromide);

- Extracting DNA from biological samples of animal origin based on phenol/chloroform;

- DNA extraction method for yeast and/or mold obtained from food based on phenol/chloroform.

For more details, see Circular 13/2013/TT-BTNMT, effective from August 5, 2013.

Le Vy

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