DNA quality and quantity determination in Vietnam

DNA quality and quantity determination in Vietnam

To be specific:, in the Technical Process and Economic-Technical Norms for detecting genetically modified organisms by qualitative and quantitative analysis methods of Deoxyribonucleic Acid issued with Circular 13/2013/TT-BTNMT, it is stipulated that the quality, quantity, and integrity of DNA samples greatly affect the results of the analysis method. According to TCVN 7606:2007 (ISO 21571), the main steps in the process of DNA quality and quantity determination include:

- Preparation of tools and chemicals;

- Electrophoresis of DNA on agarose gel

;- Determination of DNA quantity using a spectrophotometer;

- Result analysis and processing.

There are 2 common methods to determine the concentration of DNA in the solution: Electrophoresis method and spectrophotometric DNA quantity determination method.

Additionally, the PCR method is commonly used to replicate a gene segment using a primer pair specifically designed for this gene sequence. In this case, PCR is used to detect and identify genetically modified organisms, also known as qualitative PCR. According to TCVN 7605:2007 (ISO 21569), the main steps to detect and identify genetically modified organisms using PCR technique include:

- Preparation of tools and chemicals;

- Designing primer pairs to amplify the gene segment;

- Performing PCR reaction;

- Analyzing and processing results with electrophoresis on agarose gel.

For more details, refer to Circular 13/2013/TT-BTNMT, effective from August 5, 2013.

Le Vy

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