Vietnam: What are the regulations on collection of specimens for germ testing under National Technical Regulation QCVN 01- 83:2011/BNNPTNT? What are the regulations on collection of specimens for serological testing?
- What are the regulations on collection of specimens for germ testing in Vietnam under National Technical Regulation QCVN 01- 83:2011/BNNPTNT?
- What are the regulations on collection of specimens for serological testing in Vietnam?
- What are the regulations on collection of specimens for checking microscopic changes in Vietnam?
What are the regulations on collection of specimens for germ testing in Vietnam under National Technical Regulation QCVN 01- 83:2011/BNNPTNT?
In subsection 2.1 Section II of the National Technical Regulation QCVN 01- 83:2011/BNNPTNT, the collection of specimens for germ testing is specified as follows:
- Germ sampling instruments must be sterilized. The most effective conventional method is to sterilize the utensil in boiled water, the duration of which is from 15 minutes to 20 minutes, or in a specialized pot. Sampling instruments such as knives, scissors, and pint clamps must be disinfected with 70% Ethanol alcohol before and after collection of specimens.
- Have an antiseptic solution ready to wash instruments during the collection of specimens.
- Specimens taken in the body's sinuses must ensure sterility. If the specimen is used for germ culture, it must be taken immediately after surgery, the surface of the organization intended to be sterilized by high temperature (a heated blade can be applied with heating blade) and then deeply poked into the sterilization site with the implant to obtain the specimen inside that organization.
- Organ coating specimens must be taken immediately after surgery and taken in sufficient amounts from 10 grams to 200 grams (poultry taken in whole organs), separate each type of organ coating in plastic bags or sterile wide-mouth vials.
- Organ specimens with large numbers of bacteria (liver, spleen, kidney, tertiary lymphadenopathy, lungs, brain), each taken from 10 grams to 200 grams, in wide mouth vials or sterile separate plastic bags.
- For cattle, if the specimen is sent far, it is best to take additional femoral tubular bones and send them for testing.
- Use pumps, needles, or pipettes to collect heart blood, pleural fluid, coated heart, articular fluid, and cerebrospinal fluid;
Use a cotton swab to collect pus and transmission, and use a spoon to remove the contents of the intestinal tract.
- Blood or pus specimens have been fixed with methanol alcohol, and sent with other specimens to aid in laboratory diagnosis.
What are the regulations on collection of specimens for serological testing in Vietnam?
In subsection 2.2 Section II of the National Technical Regulation QCVN 01- 83:2011/BNNPTNT, the collection of specimens for serological testing in Vietnam is specified as follows:
* Blood collection location
- Buffaloes, cows, goats, and sheep use sterile needles to draw blood from the jugular vein or caudal artery.
- Pigs draw blood in the jugular vein bay, caudal artery, ear vein or iris.
- Depending on the weight of poultry to choose how to draw blood. For poultry weighing 0.5 kg or more, blood is taken in the wing vein. For poultry weighing less than 0.5 kg, blood should be taken in the jugular veins or leg veins, and heart.
- Dogs and cats draw blood from the stoop vein.
- For the recently deceased animal take heart blood
* Methods for drawing blood and draining serum
- Before taking blood from the animal to be tested, the hair must be cut on the area to be taken, disinfected with cotton alcohol 70% Ethanol, and then used a sterile needle to take from 1ml to 5ml of blood.
- The blood removed is contained in the syringe, unplugged to create a gap (or injected blood into a sterile test tube), write the sample symbol on the syringe or test tube and place it on its side 450 in the sample container, leave the blood to coagulate for 1 hour to 2 hours at normal temperature, away from direct sunlight. Then, drain the serum into another sterile test tube (or Eppendorf tube) and write the symbol of the sample on the tube containing the serum.
* Serum used to perform agglutination, precipitation, neutralization, complement combination, ELISA, and PCR.
Note: Blood should not be frozen or left outside for too long because red blood cells will destroy themselves. In case the serum sample has been centrifugally separated from all tangible components, it can be stored in the refrigerator freezer or deep freezer (freezer).
* Satisfactory serum must have a yellowish-clear color and no ruptured erythrocytes.
What are the regulations on collection of specimens for checking microscopic changes in Vietnam?
In subsection 2.4 Section II of the National Technical Regulation QCVN 01- 83:2011/BNNPTNT, the collection of specimens for checking microscopic changes is specified as follows:
- In order to observe pathological changes that are invisible to the naked eye, an optical microscope must be examined. Specimens must be selectively collected in all organs and organizations in the animal's body when conducting surgery.
+ The specimen taken must not be more than 0.5 cm thick, wide enough to determine the root organization, the two cross-sections must be parallel. The hollow organs must be open, the bones must prick out so that the fixture penetrates.
+ Specimens taken include both the general pathological transformation and the normal organization.
+ Specimens taken must not be crushed or bent.
+ Patient specimens taken from all organizations and organs in the body.
- These floating organs (lungs, bone marrow) are covered on top with a swab or cotton absorbent so that the fixture absorbs evenly. The vial containing the sample must have a wide mouth and a tight-fitting lid.
- After 24 to 48 hours, if the specimen has not been sent for testing, replace the new 10% formalin solution for further storage. When sending specimens for testing, it is only necessary to store them in a 10% Formalin solution.
- Specimens of different organizations, which can be sent in the same vial but with a vial sticker written with non-fading ink or pencil.
- Take specimens immediately after surgery; do not cool specimens before and after fixation with 10% formalin solution.
- Vials containing specimens sent for testing must be sealed against leakage, sealed, clearly labeled, easily detectable, and shatterproof; then, sent directly to a qualified diagnostic laboratory.
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