Methods for Testing Electrically-Powered Mosquito Repellent and Insecticidal Sheets

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Circular 21/2015/TT-BYT stipulates the method for testing electric mosquito repellent and killer chemical sheets by using the biological testing method in a Peet Grady chamber with dimensions 180cm x 180 cm x 180cm to examine the effectiveness, safety, and undesired effects of the chemical sheets. Each test comprises 03 trials, and all trials must adhere to the requirements, procedures, and evaluations as stipulated in this Circular.

In addition, the Circular also stipulates the requirements for this test as follows:

- Requirements for tools and equipment:- Mosquito cages made of tulle with a mesh size of 32-36 holes/cm2, dimensions of 20 cm x 20 cm x 20 cm, test cages marked in red, numbered from 01-04, and two control cages marked in blue, numbered from 01-02. The number of cages should be sufficient for the test duration;- Personal protective equipment: clothes, glasses, hats with protective nets, masks, gloves, boots;- Graph to calculate KT50, KT90;- Results recording table as specified in Appendix 1 issued with this Circular;- Questionnaire on undesired effects specified in Appendix 2 issued with this Circular;

- Technicians directly conducting the tests must be selected according to the provisions in Appendix 3 issued with this Circular.

- Mosquitoes used for testing must be female mosquitoes meeting the standards as per the guidance of the World Health Organization (WHO/CDS/CPC/MAL/98.2), bred in a Level II biosafety room, sensitive to chemicals, not blood-fed, 05 - 07 days old, fed with 10% glucose solution.

- The testing room must meet ISO/IEC 17025:2005 standards, including: 01 testing room, 01 control room according to the standards specified in Appendix 4 issued with this Circular. These rooms must have similar conditions with normal temperature and humidity, and each room should have a Peet Grady chamber with dimensions 180cm x 180 cm x 180cm. Additionally, a separate room should be prepared for observing mosquitoes' death after 24 hours with a temperature of 27°C ± 2°C and relative humidity of 80% ± 10%.

For details, refer to Circular 21/2015/TT-BYT, effective from January 01, 2016.

Le Hai

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