A.2. Physico-chemical indicators

No.

Indicator

Unit

Maximum level

Indicator category

1.

Dry matter content

%

...

...

...

A

2.

Fat content

%

≥ 3,2

A

3.

Protein content

%

...

...

...

A

4.

Density at 20 °C

g/ml

≥ 1,026

A

5.

 Titratable acidity

°T

...

...

...

A

6.

Freezing point

°C

- 0,50 to - 0,58

A

* Category A: tests are required for assessment of conformity.

APPENDIX B

...

...

...

No.

Indicator

Maximum permissible limit

CFU/ml

Indicator category

n

c

m

M

...

...

...

1.

Total aerobic plate count at 30 °C

3 x 10⁶

A

2.

Staphylococcus aureus bacteria count

5

2

500

...

...

...

A

Notes:

- n: Number of sample units to be taken from a given lot.

- c: Maximum number of sample units yielding results between m and M, unsatisfactory if total number of sample units yielding results between m and M exceed c.

- m: Limit below which all results are considered satisfactory and beyond which the results are considered satisfactory or unsatisfactory.

- M: Maximum limit which no samples are allowed to exceed.

- Category A: tests are required for assessment of conformity.

APPENDIX C

...

...

...

No.

Indicator

Maximum limit

mg/kg

Indicator category ¹⁾

1.

Lead content

0,02

A

...

...

...

APPENDIX D

TEST METHODS

No.

Indicator

Test method *

1.

Dry matter content

TCVN 8082:2013 (ISO 6731:2010) Milk, cream and evaporated milk - Determination of total solids content (Reference method)

...

...

...

Fat content

TCVN 6508:2011 (ISO 1211:2010) Milk – Determination of fat content – Gravimetric method (Reference method)

3.

Protein content

TCVN 7774:2007 (ISO 5542:1984) Milk – Determination of protein content – Amido black dye-binding method (Routine method)

TCVN 8099-1:2015 (ISO 8968-1:2014) Milk and milk products - Determination of nitrogen content - Part 1: Kjeldahl principle and crude protein calculation.

TCVN 8099-5:2009 (ISO 8968-5:2001) Milk - Determination of nitrogen content - Part 5: Determination of protein-nitrogen content

4.

Density at 20 °C

...

...

...

5.

 Titratable acidity

AOAC Official Method 947.05 Acidity of Milk. Titrimetric Method

6.

Freezing point

TCVN 7085:2011 (ISO 5764:2009) Milk - Determination of freezing point - Thermistor cryoscope method (Reference method)

7.

Somatic cells

TCVN 6686-1: 2009 (ISO 13366-1: 2008) Milk – Enumeration of somatic cells – Part 1: Microscopic method (Reference method)

...

...

...

Aflatoxin M1 content

TCVN 6685: 2009 (ISO 14501: 2007) Milk and milk powder - Determination of aflatoxin M1 content - Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

9.

Total aerobic plate count at 30 °C

TCVN 10562:2015 Foodstuffs - Enumeration of aerobic plate count using Petrifilm^TM count plate

TCVN 4884-1:2015 (ISO 4833-1:2013) Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 °C by the pour plate technique

TCVN 4884-2:2015 (ISO 4833-2:2013) Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 °C by the surface plating technique

TCVN 7923:2008 Foodstuffs - Determination of total aerobic count - Hydrophobic grid membrane filter method

10.

...

...

...

TCVN 4830-1: 2005 (ISO 6888-1: 1999, With Amd. 1:2003) Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Technique using Baird-Parker agar medium

TCVN 4830-2: 2005 (ISO 6888-2: 1999, With Amd. 1:2003) Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Technique using rabbit plasma fibrinogen agar medium

TCVN 4830-3: 2005 (ISO 6888-3: 1999, With Amd. 1:2003) Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers

11.

Lead content

TCVN 8099-1:2015 (ISO 8968-1:2014) Milk and milk products - Determination of lead content - Graphite furnace atomic absorption spectrometric method

TCVN 7602:2007 Foodstuffs – Determination of lead content by atomic absorption spectrophotometric method

TCVN 8126:2009 Foods - Determination of lead, cadmium, zinc, copper, and iron-Atomic absorption spectrophotometry after microwave digestion

TCVN 10643:2014 Foodstuffs - Determination of lead, cadmium, copper, iron, and zinc - Atomic absorption spectrophotometric method after dry ashing

...

...

...

* The above test methods or newest versions of the above test methods or test methods that have been proven equivalent to the newest versions of the above methods may be applied.